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Catalog Number | orb762424 |
---|---|
Category | Proteins |
Description | Cynomolgus IL-1 beta, His Tag is expressed from E. coli cells. It contains AA Ala 117 - Ser 268 (Accession # P79182-1). |
Target | IL-1 beta |
Conjugation | Unconjugated |
Tag | N-10×His |
Reactivity | Monkey |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | Ala 117 - Ser 268 |
UniProt ID | P79182 |
MW | 19.2 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
Expression System | E. coli |
Endotoxins | 1.0 EU per μg |
Biological Origin | Cynomolgus |
Expression Region | Ala 117 - Ser 268 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
> 97%, determined by SDS-PAGE | |
This protein contains the mature form of Cynomolgus (Macaca fascicularis) IL1B (Ala 117-Ser 269) was expressed and purified, with an initial Met. |
98.00% | |
18.33 kDa (predicted) same as Tris-Bis PAGE result. |
98.00% | |
59.47 kDa (predicted). Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |