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Cutinase Protein, Thermobifida fusca, Recombinant (His)
Catalog Number: orb1960903
| Catalog Number | orb1960903 |
|---|---|
| Category | Proteins |
| Description | Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity. |
| Tag | C-6xHis |
| Purity | 98.00% |
| MW | 28-30 KDa (reducing condition) |
| UniProt ID | E5BBQ3 |
| Protein Sequence | Ala1-Phe261 |
| Expression System | E. coli |
| Biological Origin | Thermobifida fusca |
| Biological Activity | Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity. |
| Expression Region | Ala1-Phe261 |
| Storage | -20°C |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Recombinant Thermobifida fusca Cutinase Protein, His Tag [orb1552598]
≥90% as determined by SDS-PAGE
This protein contains the Thermomonospora fusca cut-2.KW3(Ala1-Phe261) was fused with the C-terminal His Tag and expressed in E. coli.
100 μg, 50 μg
