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Catalog Number | orb753214 |
---|---|
Category | Proteins |
Description | The HEK293 derived Biotinylated recombinant protein contains the Coronavirus 2019 Spike Glycoprotein S1 Receptor Binding Domain [ RBD ], Wuhan-Hu-1 strain, amino acids 319-541 fused to His tag & AVI tag at C-terminal and having a molecular mass of 28.7kDa. |
Form/Appearance | Lyophilized freezed dried powder. |
Purity | Protein is > 90% pure as determined SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized CoV-2 S1 protein in sterile 18M-cm H2O at a concentration of 0.2mg/ml and not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions. |
Source | HEK293 Cells |
Storage | Stability: Lyophilized Cov-2 S1 Glycoprotein RBD although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CoV2 Spike protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | CoV-2 S1 RBD protein is lyophilized from 1x PBS pH-7.4 + 10% trehalose. |
Note | For research use only |
Application notes | Viral Antigens- Sars Proteins |
Expiration Date | 6 months from date of receipt. |