CNPase Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1152051
• Category: Antibodies
• Description: CNPase Recombinant Mouse Monoclonal Antibody
• Target: CNP
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG1, Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human CNPase
• UniProt ID: P09543
• MW: 48 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 7A2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 2', 3'-cyclic-nucleotide 3'-phosphodiesterase; CNP
• Research Area: Neurodegenerative Disease, Neuroglia, Neurology, N
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: 1: HeLa, 2: HEK-293, 3: Rat cerebellum, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 48 kDa, Observed MW: 48 kDa.

Cell line: SH-SY5Y, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152051.

Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-CNPase (orb1152051) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 46 kDa.

SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CNPase) polyclonal Antibody, Unconjugated (orb1152051) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.