CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb500076
• Category: Antibodies
• Description: CK10/Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody
• Target: KRT10
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human Cytokeratin 10 (550-584aa)
• UniProt ID: P13645
• MW: 59 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-10000, IHC-P=1:1000-5000, IHC-F=1:1000-5000, IF=1:1000-5000
• Antibody Type: Primary Antibody
• Clone Number: 1D8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: type I cytoskeletal 10; BCIE; BIE; CK 10; CK-10; c
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK10) Monoclonal Antibody, Unconjugated (orb500076) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CK10/Cytokeratin 10 (orb500076) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 63kD.