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Chicken Corticosterone (Cort) ELISA Kit

Catalog Number: orb782719

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb782719
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticosterone(Cort) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticosterone(Cort).
ReactivityGallus
Concentration100 ng/mL
Tested applicationsELISA
Application notesstandard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken Cort protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken Cort. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken Cort in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range1.57-100 ng/mL
Sensitivity0.5 ng/mL
Sample Typesserum, plasma, urine and other biological fluids
Alternative namesCorticosrone ELISA Kit
NoteFor research use only
Chicken Corticosterone (Cort) ELISA Kit