You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1946638 |
|---|---|
| Category | Assays and Kits |
| Description | Chicken 8-iso-PGF2α(8-isoprostane) ELISA Kit |
| Target | 8-iso-PGF2a |
| Reactivity | Gallus |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-isoprostane(8-iso-PGF2α) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-isoprostane(8-iso-PGF2α). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-isoprostane(8-iso-PGF2α) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 1.5H |
| Range | 15.625-1000 pg/mL |
| Sensitivity | 9.375 pg/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | 8-isoprostane; 8-iso-PGF2α |
| Note | For research use only |
