CD74 Antibody: Biotin

• Catalog Number: orb396381
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD74 conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6D9
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Conjugation: Biotin
• MW: 33 kDa
• Target: CD74
• Entrez: 972
• UniProt ID: P04233
• NCBI: NP_001020329.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: DHLAG antibody, HLA DR gamma antibody, HLADG antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-269 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CD74 Antibody. (D) Composite.

Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.