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Catalog Number | orb1671625 |
---|---|
Category | Antibodies |
Description | CD53 Antibody [161-2 (53/2)], Rabbit IgG |
Clonality | Recombinant |
Clone Number | 161-2 (53/2) |
Tested applications | FA, FC, IF |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was raised by immunizng mice with stimulated human leukocytes. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | CD53 |
UniProt ID | P19397 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Tetraspanin-25, Leukocyte surface antigen CD53, Ce Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of fixed Daudi cells with anti-CD53 antibody 161-2 (53/2). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of 161-2 (53/2) (orb1671625) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671625- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.