CD5 Antibody

• Catalog Number: orb1151521
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD5
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length protein corresponding to human CD5 was used as the immunogen for the recombinant CD5 antibody.
• UniProt ID: P06127
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CD5 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rC5/6429
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant CD5 antibody (clone rC5/6429) as confirmation of integrity and purity.

Western blot testing of human Jurkat cell lysate with recombinant CD5 antibody (clone rC5/6429). Observed molecular weight: 55~67 kDa depending on glycosylation level.

IHC staining of FFPE human tonsil tissue with recombinant CD5 antibody (clone rC5/6429). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant CD5 antibody (clone rC5/6429) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.