CD44 Antibody / HCAM

• Catalog Number: orb1151854
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD44
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody.
• UniProt ID: P16070
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the CD44 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rHCAM/6449
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC staining of FFPE human esophagus tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast tissue with CD44 antibody (clone rHCAM/6449). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using CD44 antibody (clone rHCAM/6449) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.