CD4 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb15274
• Category: Antibodies
• Description: CD4 Rabbit Polyclonal Antibody (FITC)
• Target: CD4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from the middle of mouse CD4 (231-330/457aa)
• RRID: AB_10746915
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 mole
• Research Area: CD Markers, Cell Biology, HIV, Immunology, Infecti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank contro: Mouse splen. Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb15274), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Dilution: 3 µg/Test. Protocol, The cells incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb15274), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb15274), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.