CD31 Antibody / PECAM-1

• Catalog Number: orb749736
• Category: Antibodies
• Description: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the CD31 antibody.
• UniProt ID: P16284
• Tested applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the PECAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: C31.10
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry test of Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled CD31 antibody (C31.10).

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD31 antibody (C31.10).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD31 antibody (C31.10).

SDS-PAGE Analysis of Purified, BSA-Free CD31 Antibody (clone C31.10). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human Jurkat cell lysate with CD31 antibody (clone C31.10). Predicted size: 83-130 kDa depending on level of glycosylation.