CD16 Rabbit Polyclonal Antibody

• Catalog Number: orb101668
• Category: Antibodies
• Description: CD16 Rabbit Polyclonal Antibody
• Target: FCGR3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human IGFR3/CD16 (131-230/254aa)
• UniProt ID: P08637
• MW: 27 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc
• Note: For research use only

Cell: U937 (4% Paraformaldehyde fixed for 10 minutes, 2% BSA at 4∑blocked for 30 minutes). Concentration: 1:100, Incubation: 40 minutes. Flow cytometric analysis of Rabbit Anti-CD16 antibody (orb101668) (green) compared with control in the absence of primary antibody (blue) followed by U937. Secondary antibody: Goat Anti-rabbit IgG/PE antibody.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD16) Polyclonal Antibody, Unconjugated (orb101668) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Thymus (Rat) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD16 (orb101668) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 60 kD.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-CD16 (orb101668) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 50 kD.