CD11a Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1152076
• Category: Antibodies
• Description: CD11a Recombinant Mouse Monoclonal Antibody
• Target: ITGAL
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human CD11a
• UniProt ID: P20701
• MW: 126 kDa
• Tested applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Clone Number: A13C2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD11a; Integrin alpha-L; Leukocyte adhesion glycop
• Research Area: Cell Biology, Erythropoiesis, Immune System, Immun
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Raji. Primary Antibody (green line): Mouse Anti-CD11a antibody (orb1152076), Dilution: 1:100, Secondary Antibody: Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152076.

Specimen: PBMC, Fixative: Unfixed, Permeabilization: None, Primary Ab Dilution: 1:100, Secondary Ab: Goat anti Mouse IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Mouse monoclonal IgG1 (Blue line). Comment: Line red is the positive signal for orb1152076.