CBFA1/RUNX2 Antibody

• Catalog Number: orb1538295
• Category: Antibodies
• Description: CBFA1/RUNX2 Antibody
• Target: CBFA1 / RUNX2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a,k
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Purification: Purified from ascites by Protein A
• Immunogen: RUNX2 (NP_004339, 251 aa ~ 351 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Application notes: Further information: Western Blot (Cell lysate) - positive control PC-12. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Clone Number: 3F5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RUNX2, AML3, CCD, CCD1, OSF2, PEBP2A1, PEBP2A2, PE
• Note: For research use only

Immunofluorescence of monoclonal antibody to RUNX2 on HeLa cell (antibody concentration 10 ug/ml).

RUNX2 monoclonal antibody, clone 3F5 Western blot of RUNX2 expression in PC-12.

Detection limit for recombinant GST tagged RUNX2 is approximately 0.1 ng/ml as a capture antibody.