Cattle Stem Cell Factor (SCF) ELISA Kit

• Catalog Number: orb779676
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor(SCF).
• Reactivity: Bovine
• Concentration: 1000 pg/mL
• UniProt ID: Q28132
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SCF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SCF in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.5 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Alternative names: KITLG ELISA Kit, MGF ELISA Kit, SF ELISA Kit, KL1
• Research Area: Hematology, Immunology & Inflammation
• Note: For research use only
• Expiration Date: Please enquire.