Cattle Hemoglobin (HB) ELISA Kit

• Catalog Number: orb782001
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin(HB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin(HB).
• Reactivity: Bovine
• Concentration: 1000 µg/mL
• Tested applications: ELISA
• Application notes: standard: 1000 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 µg/mL
• Sensitivity: 5.7 µg/mL
• Sample Types: Serum, plasma, erythrocyte lysates and other biological fluids
• Alternative names: Hgb ELISA Kit, Haemoglobin ELISA Kit, Heterotetram
• Note: For research use only