Beta-Parvin Antibody

• Catalog Number: orb1824006
• Category: Antibodies
• Description: Beta-parvin, also known as affixin, is a focal adhesion protein belonging to a family of ILKbinding proteins that contain tandem calponin homolog domains. Along with Alpha-parvin, Beta-parvin is one of the most widely researched and characterized members of this family. These two proteins interact with the ILK kinase domain in a way that is mutually exclusive and regulates both the activity of the kinase and actin cytoskeleton adhesion. Studies have shown Beta-parvin to be downregulated within human breast cancer cells, and that its expression may influence the progression of breast cancer (Johnstone, 2008). Additional research indicates a critical role for Beta-parvin in the regulation of cardiac contractility when it is in complex with ILK (Hannigan, 2007).
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PARVB/8214R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: The N-terminal region (residues 1-91) of the human protein was used as the immunogen for the Beta-Parvin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Beta-Parvin antibody is available for research use only.
• UniProt ID: Q9HBI1
• Storage: Aliquot the Beta-Parvin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human stomach tissue with Beta-Parvin antibody (clone PARVB/8214R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Parvin antibody (clone PARVB/8214R) as confirmation of integrity and purity.