Bcl-2 Antibody

• Catalog Number: orb606373
• Category: Antibodies
• Description: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.
• UniProt ID: P10415
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: rBCL2/796
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using recombinant Bcl-2 antibody (clone rBCL2/796). These results demonstrate the foremost specificity of the rBCL2/796 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human spleen tissue with recombinant Bcl-2 antibody (rBCL2/796). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl-2 antibody (clone rBCL2/796) as confirmation of integrity and purity.

Western blot testing of human MCF7 cell lysate with recombinant Bcl-2 antibody (clone rBCL2/796). Expected molecular weight ~26 kDa.

Western blot testing of human samples with recombinant Bcl-2 antibody (clone rBCL2/796). Expected molecular weight ~26 kDa.