Bax Mouse Monoclonal Antibody

• Catalog Number: orb500842
• Category: Antibodies
• Description: Bax Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8H12
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: KLH conjugated synthetic peptide derived from human Bax
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-1500, IHC-P=1:100-500, IHC-F=1:200-500, IF=1:200-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BAX
• UniProt ID: Q07812
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: apoptosis regulator BAX; Apoptosis regulator BAX c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500842) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500842) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Bax (orb500842) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.