Aurora B Antibody / AURKB

• Catalog Number: orb534225
• Category: Antibodies
• Description: Aurora B is a serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Amino acids 89-251 were used as the immunogen for this AURKB antibody.
• UniProt ID: Q96GD4
• Tested applications: IHC-P
• Dilution range: IHC (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the AURKB antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: ARRK2-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC testing of FFPE human prostate tissue with AURKB antibody (clone ARRK2-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.