ATF2 Antibody

• Catalog Number: orb2635139
• Category: Antibodies
• Description: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ATF2-1B4
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human ATF2 protein was used as the immunogen for the ATF2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ATF2 antibody is available for research use only.
• UniProt ID: P15336
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the ATF2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human HeLa cells stained using ATF2 antibody (green, clone PCRP-ATF2-1B4) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).

FACS staining of PFA-fixed human U-87 MG cells with ATF2 antibody (blue, clone PCRP-ATF2-1B4), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free ATF2 antibody (clone PCRP-ATF2-1B4) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ATF2 antibody (clone PCRP-ATF2-1B4). These results demonstrate the foremost specificity of the PCRP-ATF2-1B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.