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Catalog Number | orb152790 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against Aquaporin 1 Conjugated to RPE |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:2000), IHC (1:200), ICC/IF (1:400) |
Conjugation | RPE |
MW | 35-50kDa |
Target | Aquaporin 1 |
Entrez | 25240 |
UniProt ID | P29975 |
NCBI | NP_036910.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | AQP1 antibody, AQP 1 antibody, AQP CHIP antibody, Read more... |
Note | For research use only |
Application notes | 0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.
Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody at 1:2000. Showing glycosylated and non-glycosylated bands.