UBE2L6 Antibody

• Catalog Number: orb668554
• Category: Antibodies
• Description: The UBE2L6 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human UBE2L6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human UBE2L6. The exact sequence is proprietary.
• UniProt ID: Q9QZU9, Q4V8J2, O14933
• Tested applications: IF, WB
• Dilution range: WB: 1-500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: UBCH8; Ubiquitin/ISG15-conjugating enzyme E2 L6; R
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 56791, 295704, 9246
• Expiration Date: 12 months from date of receipt.

Western blot analysis of UBE2L6 expression in Jurkat (A), mouse lung (B), mouse spleen (C), rat kidney (D), rat liver (E) whole cell lysates. (Predicted band size: 10; 17 kD; Observed band size: 18 kD)

Immunofluorescent analysis of UBE2L6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).