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Catalog Number | orb699836 |
---|---|
Category | Antibodies |
Description | Mouse monoclonalantibody against TNAP conjugated to APC |
Clonality | Monoclonal |
Clone Number | W8B2B10 |
Tested applications | FC |
Reactivity | Canine, Goat, Human, Monkey, Porcine, Sheep |
Isotype | Mouse IgG1 |
Immunogen | WERI-RB-1 retinoblastoma cell line |
Antibody Type | Primary Antibody |
Concentration | 0.1 mg/ml |
Purity | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | APC |
Target | TNAP |
Entrez | 249 |
UniProt ID | P05186 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-Tissue Non-specific Alkaline Phosphatase anti Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 2-5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of TNAP positive HeLa cells (red-filled) from TNAP negative HeLa cells (black-dashed) in flow cytometry analysis (surface staining) of HeLa cellular suspension stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml).
Flow cytometry surface staining patterns of HeLa cells stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.56 μg/ml, same as TNAP APC concentration, right).