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Catalog Number | orb515528 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PTPN1 (phospho-Y66) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y66 of human PTP1B protein. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | PTPN1 |
Entrez | 24697, 19246, 5770 |
UniProt ID | P20417, P18031, P35821 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | PTP1B; Tyrosine-protein phosphatase non-receptor t Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PTP1B (Phospho-Y66) expression in MCF7 (A), A549 (B), mouse lung (C) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)
Direct ELISA antibody dose-response curve using Anti-PTP1B (Phospho-Y66) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.