Anti-pro-NGF beta Antibody

• Catalog Number: orb256721
• Category: Antibodies
• Description: Rabbit polyclonal antibody to pro-NGF beta
• Target: NGF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human pro-NGF beta. The exact sequence is proprietary.
• UniProt ID: P01138, P25427, P01139
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NGFB antibody, anti Beta-nerve growth factor
• Note: For research use only
• Entrez: 18049, 4803, 310738

Western blot analysis of pro-NGF beta expression in H1792 (A), mouse lung (B), rat lung (C) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)

Immunohistochemical analysis of pro-NGF beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.