Phosphotyrosine Antibody

• Catalog Number: orb44476
• Category: Antibodies
• Description: Mouse Monoclonal to Phosphotyrosine.
• Target: Phosphotyrosine
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Phosphotyrosine conjugated to bovine serum albumin.
• RRID: AB_11012581
• Tested applications: FC, ICC, WB
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 2-5 μg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: P-Tyr-01
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PY
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/ml, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 2 μg/ml, GAM FITC, black-dashed histogram).