Anti-PAK4/5 Antibody

• Catalog Number: orb737258
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PAK4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: PAK4; PAK5
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: 1/500 - 1/1000, IF: 1/100 - 1/500
• Conjugation: Unconjugated
• UniProt ID: O96013
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-KIAA1142 antibody, anti-Serine/threonine-prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PAK4/5 expression in C6 (A), CT26 (B), A2780 (C), HCT116 (D), PC3 (E) whole cell lysates.

Immunocytochemistry analysis of PAK4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.