Anti-p53 Purified

• Catalog Number: orb43682
• Category: Antibodies
• Description: Mouse monoclonal antibody to p53
• Clonality: Monoclonal
• Clone Number: BP53-12
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG2a
• Immunogen: Bacterially expressed full-length wild-type p53
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: p53
• Entrez: 7157
• UniProt ID: P04637
• RRID: AB_10995166
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti BCC7 antibody, anti FLJ92943 antibody, anti H
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml, overnight at 4°C; positive control: RAMOS human lymphoma cell line, non-reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of Ramos cells stained using anti-human p53 (BP53-12) purified antibody (concentration in sample 1.7 µg/ml, GAM APC, red-filled) from Ramos cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry (confocal microscopy) of human HeLa cells using anti-p53 (BP53-12; FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.