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Item 1 of 2
Anti-Ms MHC Class II Biotin
Catalog Number: orb738511
| Catalog Number | orb738511 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against to MHC Class II conjugated to Biotin |
| Clonality | Monoclonal |
| Clone Number | M5/114 |
| Tested applications | FC, IHC-Fr, IHC-P, IP, WB |
| Reactivity | Mouse |
| Isotype | Rat IgG2b |
| Immunogen | Activated C57BL/6 mouse spleen cells |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
| Target | MHC Class II |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 1-4 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Separation of murine MHC Class II positive splenocytes (red-filled) from MHC Class II negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/ml, Streptavidin APC).
Flow cytometry surface staining pattern of murine splenocyte suspension using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/ml, Streptavidin APC).
