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Item 1 of 2
| Catalog Number | orb338946 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to NFKBIA |
| Target | NFKBIA |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Porcine, Primate, Rat, Sheep |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y42 of human IKB alpha protein. The exact sequence is proprietary. |
| UniProt ID | Q9Z1E3, Q63746, P25963 |
| Tested applications | IH, WB |
| Dilution range | WB: 1:500-1000, IHC-P: 1:100-200 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti IKBA antibody, anti MAD3 antibody, anti NFKBI Read more... |
| Note | For research use only |
| Entrez | 18035, 25493, 4792 |
Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)
Western blot analysis of IKB alpha (Phospho-Y42) expression in K562 (A), Jurkat IL1b-treated (B), Raw264.7 LPS-treated (C), H9C2 LPS-treated (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 37 kD)
Immunohistochemical analysis of IKB alpha (Phospho-Y42) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
