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Catalog Number | orb545699 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against EpCAM conjugated to FITC |
Clonality | Monoclonal |
Clone Number | 323/A3 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Human breast cancer MCF-7 cells |
Antibody Type | Primary Antibody |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD326 |
Entrez | 4072 |
UniProt ID | P16422 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-EPCAM antibody, anti-GA733-2 antibody, anti-E Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD326 (323/A3) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension).
Flow cytometry surface staining pattern of MCF-7 cell line suspension stained using anti-human CD326 (323/A3) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension).