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Anti-Hu CD315 Purified

Catalog Number: orb1678983

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1678983
CategoryAntibodies
DescriptionCD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
ClonalityMonoclonal
Clone Number1F11
Tested applicationsFC, IHC-Fr, IP, WB
ReactivityHuman
IsotypeMouse IgG1
ImmunogenHeLa cells
Concentration1 mg/ml
ConjugationUnconjugated
TargetCD315
Entrez5738
UniProt IDQ9P2B2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Alternative namesPF2RI, SMAP-6, PTGFRN, FPRP, EWI-F
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NoteFor research use only
Application notesFlow cytometry: Recommended dilution: 1-4 µg/ml
Expiration Date12 months from date of receipt.
Anti-Hu CD315 Purified

Separation of A431 cells stained using anti-human CD315 (1F11) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from A431 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).