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Catalog Number | orb339062 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to HMGB1 |
Target | HMGB1 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Porcine, Primate, Rat |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Immunogen | KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K12 of human HMGB1 protein. The exact sequence is proprietary. |
UniProt ID | P09429, P63158, P63159 |
Tested applications | WB |
Dilution range | WB: 1:500-1000 |
Antibody Type | Primary Antibody |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti HMG1 antibody, anti High mobility group prote Read more... |
Note | For research use only |
Entrez | 100862258, 25459, 15289, 3146 |
Western blot analysis of HMGB1 (Acetyl-K12) expression in DLD (A), rat liver (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
Direct ELISA antibody dose-response curve using Anti-HMGB1 (Acetyl-K12) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.