HLA-E Antibody

• Catalog Number: orb44423
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-E.
• Target: HLA-E
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
• UniProt ID: P13747
• RRID: AB_10991868
• Tested applications: FC, IHC-P, IP
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Antibody Type: Primary Antibody
• Clone Number: MEM-E/07
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HLA class I histocompatibility antigen, alpha chai
• Research Area: Epigenetics, Immunology, Infectious Diseases
• Note: For research use only
• Entrez: 3133

Separation of HLA-E transfected LCL cells stained using anti-human HLA-E (MEM-E/07) purified antibody (concentration in sample 2 µg/ml, red-filled) from HLA-E transfected LCL cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 2 µg/ml, same as HLA-E purified antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).