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Anti-Cytokeratin 10/13 Purified
Catalog Number: orb43753
| Catalog Number | orb43753 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Cytokeratin 10/13. |
| Clonality | Monoclonal |
| Clone Number | DE-K13 |
| Tested applications | IHC-Fr, IHC-P, WB |
| Reactivity | Feline, Human |
| Isotype | Mouse IgG2a |
| Immunogen | Cytoskeletal preparation extracted from human ectocervical epithelium. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | Cytokeratin 10/13 |
| RRID | AB_10992492 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-Cytokeratin 10+13 antibody Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections): Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-Cytokeratin 10+13, (DE-K13). The antibody DE-K13 stains only Cytokeratin 13 when used on formalin-fixed, paraffin-embedded tissue sections.
