Anti-CEACAM5 antibody(DM120); Rabbit mAb

• Catalog Number: orb1174048
• Category: Antibodies
• Description: Anti-CEACAM5 antibody(DM120), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM120
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CEACAM5
• UniProt ID: P06731
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CEACAM-5;CD66e;CEA;Meconium antigen 100
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human CEACAM5 protein, His tagged protein orb689419 can bind Rabbit anti-CEACAM5 monoclonal antibody (clone: DM120) in a linear range of 0.1-50 ng/ml.

Flow cytometry analysis with Anti-CEACAM5 on Expi293 cells transfected with human CEACAM5 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human CEACAM5 mAb (orb1174048). (A) orb1174048 does not bind to 293T cells that do not express CEACAM5. (B) A clear peak shift of orb1174048 was seen compared to the control when incubated with CEACAM5-expressing HT55 cells, indicating strong binding of orb1174048 to CEACAM5. Antibodies were incubated at 2 μg/mL.