Anti-CEA [T84.66]

• Catalog Number: orb2645808
• Category: Antibodies
• Description: Anti-CEA [T84.66]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: T84.66
• Tested applications: ELISA, FC, IHC, RIA
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: CEA
• UniProt ID: P06731
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Carcinoembryonic antigen; Carcinoembryonic antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MCF7 cells with anti-CEA antibody T84.66. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of T84.66 (orb2645808) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645808, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-CEA antibody T84.66. MCF7 (A) (0.03 µg/ml) cells lysate, human colon (B) (0.03 µg/ml) tissue lysate, and human colorectal carcinoma (C) (0.0003 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of T84.66 (orb2645808) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.