Anti-CEA [EB-022]

• Catalog Number: orb1463372
• Category: Antibodies
• Description: Mouse monoclonal antibody to CEA
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EB-022
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This antibody was generated by immunising BALB/c mice. Splenocytes from immunised mice were obtained and fused with myeloma cells to generate a hybridoma.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CEA
• UniProt ID: P06731
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Carcinoembryonic antigen; Carcinoembryonic antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MCF7 cells with anti-CEA EB-022. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (orb1463371) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463371, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CEA antibody EB-022. Human colorectal carcinoma (0.01 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of EB-022 (orb1463371) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.