Anti-CD16/CD32 [2.4G2]

• Catalog Number: orb348903
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD16/CD32
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 2.4G2
• Tested applications: Blocking, FC, IF, IHC, IP
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: Mouse BALB/c Macrophage J774 Cell Line.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD16/CD32
• UniProt ID: P08508, P08101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fcγ III/II Receptor; Low affinity immunoglobulin g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Unkeless, J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors J Exp Med, 150, 580-596 (1979)

PubMed: 90108

Anti-CD16/32 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody orb314897 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647 (AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/32-positive population is apparent.

Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (orb314897, clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.