BTK (Phospho-Y223) Antibody

• Catalog Number: orb216039
• Category: Antibodies
• Description: The BTK (Phospho-Y223) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y223 of human BTK protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y223 of human BTK protein. The exact sequence is proprietary.
• UniProt ID: P35991, Q06187
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agam
• Research Area: Apoptotic, Cancer, Epigenetics, Immunology, Neuros
• Note: For research use only
• Entrez: 695, 12229

Western blot analysis of BTK (Phospho-Y223) expression in mouse spleen (A), mouse liver (B) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 75 kD)

Immunofluorescent analysis of BTK (Phospho-Y223) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-BTK (Phospho-Y223) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.