Anti-B7-H3 [MJ18]

• Catalog Number: orb613951
• Category: Antibodies
• Description: Rat monoclonal antibody to B7-H3
• Target: B7-H3
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1–242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
• UniProt ID: Q8VE98
• Tested applications: Blocking, FC
• Clone Number: MJ18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD276; B7H3
• Note: For research use only

Binding curve of anti-B7-H3 antibody MJ18 (orb613950) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613950. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (orb613950) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613950, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody