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Anti-APPBP1/NAE1 Antibody
Catalog Number: orb251522
| Catalog Number | orb251522 |
|---|---|
| Category | Antibodies |
| Description | Anti-APPBP1/NAE1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human APPBP1 recombinant protein (Position: E181-L534). Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 42 kDa |
| UniProt ID | Q13564 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | NEDD8-activating enzyme E1 regulatory subunit; Amy Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of APPBP1/NAE1 using anti-APPBP1/NAE1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPBP1/NAE1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APPBP1/NAE1 at approximately 60 kDa. The expected band size for APPBP1/NAE1 is at 60 kDa.
