alpha-Fetoprotein Antibody (Biotin)

• Catalog Number: orb1480515
• Category: Antibodies
• Description: Mouse monoclonal antibody against alpha-Fetoprotein conjugated to Biotin
• Target: alpha-Fetoprotein
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Immunogen: purified alpha-Fetoprotein
• UniProt ID: P02771
• Tested applications: ELISA, ICC, IP, WB
• Application notes: ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
• Antibody Type: Primary Antibody
• Clone Number: AFP-01
• Storage: Store at 2-8°C. Do not freeze.
• Alternative names: AFP, FETA, HPAFP
• Note: For research use only
• Entrez: 174

ELISA analysis of alpha-fetoprotein (AFP) using biotin-conjugated detection antibody AFP-01. Different alpha-fetoprotein concentrations (0 - 5 μg/ml) and different detection antibody concentrations (0.016 - 10 μg/ml) were tested.