Alpha-Tubulin Antibody Biotin Conjugated

• Catalog Number: orb345885
• Category: Antibodies
• Description: Tubulin alpha antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Bovine, Gallus, Goat, Human, Mouse, Rat, Sheep
• Form/Appearance: Lyophilized
• Concentration: 0.61 mg/ml
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
• UniProt ID: P68363
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, IF: 1:500 - 1:2,000, WB: 1:2,000-1:10,000
• Application notes: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.
• Antibody Type: Primary Antibody
• Storage: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-alpha-Tubulin Antibody biotin conjugat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Results of Purified Rabbit Anti-Alpha Tubulin Antibody Biotin Conjugated tested against purified Alpha Tubulin Biotin Conjugated. Each well was coated in duplicate with 0.1 µg of BSA conjugated Alpha Tubulin. The working dilution is 1:11000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).