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Item 1 of 3
Alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1152016
| Catalog Number | orb1152016 |
|---|---|
| Category | Antibodies |
| Description | Alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody |
| Target | ACTN4 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Mouse |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human alpha Actinin 4 |
| UniProt ID | O43707 |
| MW | 105 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:200-1000, IHC-P=1:20-1:100, IHC-F=1:400-800, ICC/IF=1:20-1:100, IF=1:20-1:100 |
| Antibody Type | Recombinant Antibody |
| Clone Number | A12H9 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | actinin 4; Actinin alpha 4; Actinin alpha-4; alpha Read more... |
| Note | For research use only |
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152016.
Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-alpha Actinin 4 (orb1152016) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kDa, Observed band size: 108 kDa.
Tissue: Human colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152016.
