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Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Donkey]

Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Donkey]

Catalog Number: orb1675031

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1675031
CategoryAntibodies
DescriptionMinimum Cross reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins
Species/HostDonkey
Tested applicationsDOT, ELISA, IHC, WB
ReactivityRabbit
ImmunogenRabbit IgG whole molecule
Antibody TypeSecondary Antibody
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
ConjugationUnconjugated
StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Buffer/Preservatives0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
NoteFor research use only
Application notesELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 1:400 - 1:2,000
Expiration Date12 months from date of receipt.