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Catalog Number | orb1675031 |
---|---|
Category | Antibodies |
Description | Minimum Cross reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins |
Species/Host | Donkey |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Rabbit |
Immunogen | Rabbit IgG whole molecule |
Antibody Type | Secondary Antibody |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. |
Conjugation | Unconjugated |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product. |
Buffer/Preservatives | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Note | For research use only |
Application notes | ELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:1,000 - 1:5,000 IMMUNOHISTOCHEMISTRY 1:400 - 1:2,000 |
Expiration Date | 12 months from date of receipt. |