AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499280
• Category: Antibodies
• Description: AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody
• Target: AKT2
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human AKT1 / AKT2 / AKT3
• UniProt ID: P31751
• MW: 56 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:200-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: R2A4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AKT1(phospho T308); AKT2(phospho T309); AKT3(phosp
• Research Area: Akt/PKB Signaling Pathway, CARdiovascular Research
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: HeLa, 3: Mouse brain, 4: Rat brain, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 56 kDa, Observed MW: 56 kDa.

Tissue: Mouse cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499280.

Tissue: Rat cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499280.